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As I assume, it should make no visual difference if duplicate a layer and then show both layer using normal?

forgot to mention, both layers have opacity setting 100%

enter image description here

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up vote 3 down vote accepted

Actually there will be a difference if the image has translucent pixels. Pixel build-up will cause some pixels to appear darker.

Stack an 80% opaque pixel on top of another 80% opaque pixel and things will look 100% opaque.

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