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This question already has an answer here:

After you've made the background transparent, color selected the background and inverted the selection to outline all the character sprites:

Is there a shortcut or some quicker method/program/tool to turn each sprite into an individual selection and then turn each selection into an individual layer? If not with GIMP, are there any other free programs that will allow you to do this?

marked as duplicate by go-junta, Hanna, Zach Saucier, Wrzlprmft, Ryan Sep 21 '15 at 10:46

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    Be more specific, please. Is there a sort of regularity for your sprites (e.g. equidistant spacing or similar)? If yes, you can use ImageMagick to split the image into tiles. – Paolo Gibellini Oct 21 '14 at 19:50
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    See also here – Paolo Gibellini Nov 12 '14 at 14:28
  • Are you trying to get all of your sprites as layers? For example, you start with a 100x100 image with 25 sprites that are 20x20, and you want to end up with a 20x20 image with 25 layers, one sprite per layer. I'm curious what the usefulness of that would be, but if that's what you want to do, splitting to image into individual files per sprite, then importing as layers is probably the fastest. – Scribblemacher Jul 27 '15 at 12:08