I was enjoying the use of different transparency settings in both programs and was wondering if the simulated transparency would have any effect on the use of the images in different production environments(web and print)?
Here is the answer: It depends ;)
Apart from your confusion between alpha and pixel density, The possibilities are quite vast so there is no real answer. It depends on many things like which color profile is used and if you have a consistent color setting in your programs.
In web the big fun begins when you realize that not al browsers support color profiles.
So there is only one real solution: Test it yourself.